a pchk1 s317 rabbit cell signaling tech (Cell Signaling Technology Inc)
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Cell Signaling Technology Inc
a pchk1 s317 rabbit cell signaling tech
A Pchk1 S317 Rabbit Cell Signaling Tech, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 636 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pchk1+antibody/Phospho-Chk1+(Ser317)+Antibody/pm41914023-71-40-43
Average 96 stars, based on 636 article reviews
A Pchk1 S317 Rabbit Cell Signaling Tech, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 636 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pchk1+antibody/Phospho-Chk1+(Ser317)+Antibody/pm41914023-71-40-43
Average 96 stars, based on 636 article reviews
a pchk1 s317 rabbit cell signaling tech - by Bioz Stars,
2026-10
96/100 stars
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Recombinant:Article Title: ZEB1 loss increases glioma stem cell tumorigenicity and resistance to chemoradiation Article Snippet: Glioblastoma (GBM) is associated with short survival, largely due to GBM resistance to existing therapies.. Even after surgical resection, chemoradiation treatment only increases survival from 12 months to 15 months.1 Much of the research evaluating the mechanisms surrounding both chemotherapy and radiation resistance has focused on the identification of a subset of cancer stem cells within GBMs, i.e., the glioma stem cells (GSCs).2–5 The GSCs have long been thought to be responsible for conferring chemoand radioresistance resulting in disease progression and shortened survival in GBM patients.6–9 Given that chemotherapy in conjunction with radiation plays a large role in increasing survival,10 we looked for other potential targets that may play a role in both chemotherapy and radiation resistance in GSCs.. The identification of targetable markers in GSCs has a potential therapeutic role, as shown recently by Putthisen et al., who demonstrated that GSC-associated sialic acid–modified glycan with Maackia amurensis lectin II (MAL-II)– binding alpha2,3-sialylated glycan (MAL-SG) has a role Article Title: Targeting the ATR/CHK1 Axis with PARP Inhibition Results in Tumor Regression in BRCA-Mutant Ovarian Cancer Models Article Snippet: Slides were incubated with Article Title: Removal of uracil by uracil DNA glycosylase limits pemetrexed cytotoxicity: overriding the limit with methoxyamine to inhibit base excision repair Article Snippet: Sources of primary antibody were as follows: cleaved PARP (BD Pharmingen, San Jose, CA, USA), γ H2AX (Bethyl), pChk1, Chk1, pcdc2, cyclin B1, topo II α , topoisomerase I, Bax, and Article Title: Reactive Oxygen Species-independent Oxidation of Thioredoxin in Hypoxia Article Snippet: The antibodies used in our study were obtained from the following vendors: Trx, p21, and Chk1 antibodies were obtained from Santa Cruz Biotechnology (Santa Cruz, CA); RNR, MnSOD, and Chk2 antibodies were obtained from Millipore (Billerica, MA); poly(ADP-ribose) polymerase (PARP), p53, Article Title: Biphasic response of checkpoint control proteins in hyperoxia: Exposure to lower levels of oxygen induces genome maintenance genes in experimental baboon BPD Article Snippet: Chk2 antibody was obtained from Millipore (Billerica, MA); poly-ADP ribose polymerase (PARP), p53, phospho-p53 (ser15), and Article Title: Phosphorylation of nuclear Tau is modulated by distinct cellular pathways Article Snippet: Primary antibodies, usually incubated for 1 h at 37 °C, were specific for human Tau (Tau13, Santa Cruz, sc-21796, used at 1 μg/mL), GFP (Proteintech Europe, 66002–1-Ig, 7 μg/mL), calnexin (kind gift of Prof. Maurizio Molinari, IRB, Bellinzona, Switzerland, diluted 1:1,000), α-tubulin (Abcam, ab1825, 0.5 μg/mL or Cell Signaling, DM1A, diluted 1:500), pS 129 -H2A.X (Santa Cruz, sc-517348, 0.5 μg/mL), Article Title: Natural bioactive gallic acid shows potential anticancer effects by inhibiting the proliferation and invasiveness behavior in human embryonic carcinoma cells Article Snippet: Article Title: Replication stress and defective checkpoints make fallopian tube epithelial cells putative drivers of high-grade serous ovarian cancer. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies gH2AX Millipore Cat# 05-636; RRID: AB_309864 PAX8 Proteintech Cat# 10336; RRID: AB_2918972 CK8 TROMA-I -DSHB Cat# AB_531826; RRID: AB_531826 53BP1 Bethyl Labs Cat# A300-272A; RRID:AB_185520 53BP1 BioLegend Cat# 933002; RRID:AB_2820202 Foxj1 Millipore Sigma Cat# HPA005714; RRID:AB_1078902 Rad51 Santa Cruz Cat# SC-8349; RRID:AB_2253533 pRPA32 (S4/S8) Bethyl Labs Cat# A300-245A; RRID:AB_210547 pRPA32 (S33) Bethyl Labs Cat# Polymer:Article Title: ZEB1 loss increases glioma stem cell tumorigenicity and resistance to chemoradiation Article Snippet: Glioblastoma (GBM) is associated with short survival, largely due to GBM resistance to existing therapies.. Even after surgical resection, chemoradiation treatment only increases survival from 12 months to 15 months.1 Much of the research evaluating the mechanisms surrounding both chemotherapy and radiation resistance has focused on the identification of a subset of cancer stem cells within GBMs, i.e., the glioma stem cells (GSCs).2–5 The GSCs have long been thought to be responsible for conferring chemoand radioresistance resulting in disease progression and shortened survival in GBM patients.6–9 Given that chemotherapy in conjunction with radiation plays a large role in increasing survival,10 we looked for other potential targets that may play a role in both chemotherapy and radiation resistance in GSCs.. The identification of targetable markers in GSCs has a potential therapeutic role, as shown recently by Putthisen et al., who demonstrated that GSC-associated sialic acid–modified glycan with Maackia amurensis lectin II (MAL-II)– binding alpha2,3-sialylated glycan (MAL-SG) has a role Article Title: Targeting the ATR/CHK1 Axis with PARP Inhibition Results in Tumor Regression in BRCA-Mutant Ovarian Cancer Models Article Snippet: Slides were incubated with Article Title: Removal of uracil by uracil DNA glycosylase limits pemetrexed cytotoxicity: overriding the limit with methoxyamine to inhibit base excision repair Article Snippet: Sources of primary antibody were as follows: cleaved PARP (BD Pharmingen, San Jose, CA, USA), γ H2AX (Bethyl), pChk1, Chk1, pcdc2, cyclin B1, topo II α , topoisomerase I, Bax, and Article Title: Reactive Oxygen Species-independent Oxidation of Thioredoxin in Hypoxia Article Snippet: The antibodies used in our study were obtained from the following vendors: Trx, p21, and Chk1 antibodies were obtained from Santa Cruz Biotechnology (Santa Cruz, CA); RNR, MnSOD, and Chk2 antibodies were obtained from Millipore (Billerica, MA); poly(ADP-ribose) polymerase (PARP), p53, Article Title: Biphasic response of checkpoint control proteins in hyperoxia: Exposure to lower levels of oxygen induces genome maintenance genes in experimental baboon BPD Article Snippet: Chk2 antibody was obtained from Millipore (Billerica, MA); poly-ADP ribose polymerase (PARP), p53, phospho-p53 (ser15), and Article Title: Phosphorylation of nuclear Tau is modulated by distinct cellular pathways Article Snippet: Primary antibodies, usually incubated for 1 h at 37 °C, were specific for human Tau (Tau13, Santa Cruz, sc-21796, used at 1 μg/mL), GFP (Proteintech Europe, 66002–1-Ig, 7 μg/mL), calnexin (kind gift of Prof. Maurizio Molinari, IRB, Bellinzona, Switzerland, diluted 1:1,000), α-tubulin (Abcam, ab1825, 0.5 μg/mL or Cell Signaling, DM1A, diluted 1:500), pS 129 -H2A.X (Santa Cruz, sc-517348, 0.5 μg/mL), Article Title: Natural bioactive gallic acid shows potential anticancer effects by inhibiting the proliferation and invasiveness behavior in human embryonic carcinoma cells Article Snippet: Article Title: Replication stress and defective checkpoints make fallopian tube epithelial cells putative drivers of high-grade serous ovarian cancer. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies gH2AX Millipore Cat# 05-636; RRID: AB_309864 PAX8 Proteintech Cat# 10336; RRID: AB_2918972 CK8 TROMA-I -DSHB Cat# AB_531826; RRID: AB_531826 53BP1 Bethyl Labs Cat# A300-272A; RRID:AB_185520 53BP1 BioLegend Cat# 933002; RRID:AB_2820202 Foxj1 Millipore Sigma Cat# HPA005714; RRID:AB_1078902 Rad51 Santa Cruz Cat# SC-8349; RRID:AB_2253533 pRPA32 (S4/S8) Bethyl Labs Cat# A300-245A; RRID:AB_210547 pRPA32 (S33) Bethyl Labs Cat# Incubation:Article Title: ZEB1 loss increases glioma stem cell tumorigenicity and resistance to chemoradiation Article Snippet: Glioblastoma (GBM) is associated with short survival, largely due to GBM resistance to existing therapies.. Even after surgical resection, chemoradiation treatment only increases survival from 12 months to 15 months.1 Much of the research evaluating the mechanisms surrounding both chemotherapy and radiation resistance has focused on the identification of a subset of cancer stem cells within GBMs, i.e., the glioma stem cells (GSCs).2–5 The GSCs have long been thought to be responsible for conferring chemoand radioresistance resulting in disease progression and shortened survival in GBM patients.6–9 Given that chemotherapy in conjunction with radiation plays a large role in increasing survival,10 we looked for other potential targets that may play a role in both chemotherapy and radiation resistance in GSCs.. The identification of targetable markers in GSCs has a potential therapeutic role, as shown recently by Putthisen et al., who demonstrated that GSC-associated sialic acid–modified glycan with Maackia amurensis lectin II (MAL-II)– binding alpha2,3-sialylated glycan (MAL-SG) has a role Article Title: Targeting the ATR/CHK1 Axis with PARP Inhibition Results in Tumor Regression in BRCA-Mutant Ovarian Cancer Models Article Snippet: Slides were incubated with Article Title: Removal of uracil by uracil DNA glycosylase limits pemetrexed cytotoxicity: overriding the limit with methoxyamine to inhibit base excision repair Article Snippet: Sources of primary antibody were as follows: cleaved PARP (BD Pharmingen, San Jose, CA, USA), γ H2AX (Bethyl), pChk1, Chk1, pcdc2, cyclin B1, topo II α , topoisomerase I, Bax, and Article Title: Reactive Oxygen Species-independent Oxidation of Thioredoxin in Hypoxia Article Snippet: The antibodies used in our study were obtained from the following vendors: Trx, p21, and Chk1 antibodies were obtained from Santa Cruz Biotechnology (Santa Cruz, CA); RNR, MnSOD, and Chk2 antibodies were obtained from Millipore (Billerica, MA); poly(ADP-ribose) polymerase (PARP), p53, Article Title: Biphasic response of checkpoint control proteins in hyperoxia: Exposure to lower levels of oxygen induces genome maintenance genes in experimental baboon BPD Article Snippet: Chk2 antibody was obtained from Millipore (Billerica, MA); poly-ADP ribose polymerase (PARP), p53, phospho-p53 (ser15), and Article Title: Phosphorylation of nuclear Tau is modulated by distinct cellular pathways Article Snippet: Primary antibodies, usually incubated for 1 h at 37 °C, were specific for human Tau (Tau13, Santa Cruz, sc-21796, used at 1 μg/mL), GFP (Proteintech Europe, 66002–1-Ig, 7 μg/mL), calnexin (kind gift of Prof. Maurizio Molinari, IRB, Bellinzona, Switzerland, diluted 1:1,000), α-tubulin (Abcam, ab1825, 0.5 μg/mL or Cell Signaling, DM1A, diluted 1:500), pS 129 -H2A.X (Santa Cruz, sc-517348, 0.5 μg/mL), Article Title: Natural bioactive gallic acid shows potential anticancer effects by inhibiting the proliferation and invasiveness behavior in human embryonic carcinoma cells Article Snippet: Article Title: Replication stress and defective checkpoints make fallopian tube epithelial cells putative drivers of high-grade serous ovarian cancer. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies gH2AX Millipore Cat# 05-636; RRID: AB_309864 PAX8 Proteintech Cat# 10336; RRID: AB_2918972 CK8 TROMA-I -DSHB Cat# AB_531826; RRID: AB_531826 53BP1 Bethyl Labs Cat# A300-272A; RRID:AB_185520 53BP1 BioLegend Cat# 933002; RRID:AB_2820202 Foxj1 Millipore Sigma Cat# HPA005714; RRID:AB_1078902 Rad51 Santa Cruz Cat# SC-8349; RRID:AB_2253533 pRPA32 (S4/S8) Bethyl Labs Cat# A300-245A; RRID:AB_210547 pRPA32 (S33) Bethyl Labs Cat# Staining:Article Title: ZEB1 loss increases glioma stem cell tumorigenicity and resistance to chemoradiation Article Snippet: Glioblastoma (GBM) is associated with short survival, largely due to GBM resistance to existing therapies.. Even after surgical resection, chemoradiation treatment only increases survival from 12 months to 15 months.1 Much of the research evaluating the mechanisms surrounding both chemotherapy and radiation resistance has focused on the identification of a subset of cancer stem cells within GBMs, i.e., the glioma stem cells (GSCs).2–5 The GSCs have long been thought to be responsible for conferring chemoand radioresistance resulting in disease progression and shortened survival in GBM patients.6–9 Given that chemotherapy in conjunction with radiation plays a large role in increasing survival,10 we looked for other potential targets that may play a role in both chemotherapy and radiation resistance in GSCs.. The identification of targetable markers in GSCs has a potential therapeutic role, as shown recently by Putthisen et al., who demonstrated that GSC-associated sialic acid–modified glycan with Maackia amurensis lectin II (MAL-II)– binding alpha2,3-sialylated glycan (MAL-SG) has a role Article Title: Targeting the ATR/CHK1 Axis with PARP Inhibition Results in Tumor Regression in BRCA-Mutant Ovarian Cancer Models Article Snippet: Slides were incubated with Article Title: Removal of uracil by uracil DNA glycosylase limits pemetrexed cytotoxicity: overriding the limit with methoxyamine to inhibit base excision repair Article Snippet: Sources of primary antibody were as follows: cleaved PARP (BD Pharmingen, San Jose, CA, USA), γ H2AX (Bethyl), pChk1, Chk1, pcdc2, cyclin B1, topo II α , topoisomerase I, Bax, and Article Title: Reactive Oxygen Species-independent Oxidation of Thioredoxin in Hypoxia Article Snippet: The antibodies used in our study were obtained from the following vendors: Trx, p21, and Chk1 antibodies were obtained from Santa Cruz Biotechnology (Santa Cruz, CA); RNR, MnSOD, and Chk2 antibodies were obtained from Millipore (Billerica, MA); poly(ADP-ribose) polymerase (PARP), p53, Article Title: Biphasic response of checkpoint control proteins in hyperoxia: Exposure to lower levels of oxygen induces genome maintenance genes in experimental baboon BPD Article Snippet: Chk2 antibody was obtained from Millipore (Billerica, MA); poly-ADP ribose polymerase (PARP), p53, phospho-p53 (ser15), and Article Title: Phosphorylation of nuclear Tau is modulated by distinct cellular pathways Article Snippet: Primary antibodies, usually incubated for 1 h at 37 °C, were specific for human Tau (Tau13, Santa Cruz, sc-21796, used at 1 μg/mL), GFP (Proteintech Europe, 66002–1-Ig, 7 μg/mL), calnexin (kind gift of Prof. Maurizio Molinari, IRB, Bellinzona, Switzerland, diluted 1:1,000), α-tubulin (Abcam, ab1825, 0.5 μg/mL or Cell Signaling, DM1A, diluted 1:500), pS 129 -H2A.X (Santa Cruz, sc-517348, 0.5 μg/mL), Article Title: Natural bioactive gallic acid shows potential anticancer effects by inhibiting the proliferation and invasiveness behavior in human embryonic carcinoma cells Article Snippet: Article Title: Replication stress and defective checkpoints make fallopian tube epithelial cells putative drivers of high-grade serous ovarian cancer. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies gH2AX Millipore Cat# 05-636; RRID: AB_309864 PAX8 Proteintech Cat# 10336; RRID: AB_2918972 CK8 TROMA-I -DSHB Cat# AB_531826; RRID: AB_531826 53BP1 Bethyl Labs Cat# A300-272A; RRID:AB_185520 53BP1 BioLegend Cat# 933002; RRID:AB_2820202 Foxj1 Millipore Sigma Cat# HPA005714; RRID:AB_1078902 Rad51 Santa Cruz Cat# SC-8349; RRID:AB_2253533 pRPA32 (S4/S8) Bethyl Labs Cat# A300-245A; RRID:AB_210547 pRPA32 (S33) Bethyl Labs Cat# Microscopy:Article Title: ZEB1 loss increases glioma stem cell tumorigenicity and resistance to chemoradiation Article Snippet: Glioblastoma (GBM) is associated with short survival, largely due to GBM resistance to existing therapies.. Even after surgical resection, chemoradiation treatment only increases survival from 12 months to 15 months.1 Much of the research evaluating the mechanisms surrounding both chemotherapy and radiation resistance has focused on the identification of a subset of cancer stem cells within GBMs, i.e., the glioma stem cells (GSCs).2–5 The GSCs have long been thought to be responsible for conferring chemoand radioresistance resulting in disease progression and shortened survival in GBM patients.6–9 Given that chemotherapy in conjunction with radiation plays a large role in increasing survival,10 we looked for other potential targets that may play a role in both chemotherapy and radiation resistance in GSCs.. The identification of targetable markers in GSCs has a potential therapeutic role, as shown recently by Putthisen et al., who demonstrated that GSC-associated sialic acid–modified glycan with Maackia amurensis lectin II (MAL-II)– binding alpha2,3-sialylated glycan (MAL-SG) has a role Article Title: Targeting the ATR/CHK1 Axis with PARP Inhibition Results in Tumor Regression in BRCA-Mutant Ovarian Cancer Models Article Snippet: Slides were incubated with Article Title: Removal of uracil by uracil DNA glycosylase limits pemetrexed cytotoxicity: overriding the limit with methoxyamine to inhibit base excision repair Article Snippet: Sources of primary antibody were as follows: cleaved PARP (BD Pharmingen, San Jose, CA, USA), γ H2AX (Bethyl), pChk1, Chk1, pcdc2, cyclin B1, topo II α , topoisomerase I, Bax, and Article Title: Reactive Oxygen Species-independent Oxidation of Thioredoxin in Hypoxia Article Snippet: The antibodies used in our study were obtained from the following vendors: Trx, p21, and Chk1 antibodies were obtained from Santa Cruz Biotechnology (Santa Cruz, CA); RNR, MnSOD, and Chk2 antibodies were obtained from Millipore (Billerica, MA); poly(ADP-ribose) polymerase (PARP), p53, Article Title: Biphasic response of checkpoint control proteins in hyperoxia: Exposure to lower levels of oxygen induces genome maintenance genes in experimental baboon BPD Article Snippet: Chk2 antibody was obtained from Millipore (Billerica, MA); poly-ADP ribose polymerase (PARP), p53, phospho-p53 (ser15), and Article Title: Phosphorylation of nuclear Tau is modulated by distinct cellular pathways Article Snippet: Primary antibodies, usually incubated for 1 h at 37 °C, were specific for human Tau (Tau13, Santa Cruz, sc-21796, used at 1 μg/mL), GFP (Proteintech Europe, 66002–1-Ig, 7 μg/mL), calnexin (kind gift of Prof. Maurizio Molinari, IRB, Bellinzona, Switzerland, diluted 1:1,000), α-tubulin (Abcam, ab1825, 0.5 μg/mL or Cell Signaling, DM1A, diluted 1:500), pS 129 -H2A.X (Santa Cruz, sc-517348, 0.5 μg/mL), Article Title: Natural bioactive gallic acid shows potential anticancer effects by inhibiting the proliferation and invasiveness behavior in human embryonic carcinoma cells Article Snippet: Article Title: Replication stress and defective checkpoints make fallopian tube epithelial cells putative drivers of high-grade serous ovarian cancer. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies gH2AX Millipore Cat# 05-636; RRID: AB_309864 PAX8 Proteintech Cat# 10336; RRID: AB_2918972 CK8 TROMA-I -DSHB Cat# AB_531826; RRID: AB_531826 53BP1 Bethyl Labs Cat# A300-272A; RRID:AB_185520 53BP1 BioLegend Cat# 933002; RRID:AB_2820202 Foxj1 Millipore Sigma Cat# HPA005714; RRID:AB_1078902 Rad51 Santa Cruz Cat# SC-8349; RRID:AB_2253533 pRPA32 (S4/S8) Bethyl Labs Cat# A300-245A; RRID:AB_210547 pRPA32 (S33) Bethyl Labs Cat# Western Blot:Article Title: ZEB1 loss increases glioma stem cell tumorigenicity and resistance to chemoradiation Article Snippet: Glioblastoma (GBM) is associated with short survival, largely due to GBM resistance to existing therapies.. Even after surgical resection, chemoradiation treatment only increases survival from 12 months to 15 months.1 Much of the research evaluating the mechanisms surrounding both chemotherapy and radiation resistance has focused on the identification of a subset of cancer stem cells within GBMs, i.e., the glioma stem cells (GSCs).2–5 The GSCs have long been thought to be responsible for conferring chemoand radioresistance resulting in disease progression and shortened survival in GBM patients.6–9 Given that chemotherapy in conjunction with radiation plays a large role in increasing survival,10 we looked for other potential targets that may play a role in both chemotherapy and radiation resistance in GSCs.. The identification of targetable markers in GSCs has a potential therapeutic role, as shown recently by Putthisen et al., who demonstrated that GSC-associated sialic acid–modified glycan with Maackia amurensis lectin II (MAL-II)– binding alpha2,3-sialylated glycan (MAL-SG) has a role Article Title: Targeting the ATR/CHK1 Axis with PARP Inhibition Results in Tumor Regression in BRCA-Mutant Ovarian Cancer Models Article Snippet: Slides were incubated with Article Title: Removal of uracil by uracil DNA glycosylase limits pemetrexed cytotoxicity: overriding the limit with methoxyamine to inhibit base excision repair Article Snippet: Sources of primary antibody were as follows: cleaved PARP (BD Pharmingen, San Jose, CA, USA), γ H2AX (Bethyl), pChk1, Chk1, pcdc2, cyclin B1, topo II α , topoisomerase I, Bax, and Article Title: Reactive Oxygen Species-independent Oxidation of Thioredoxin in Hypoxia Article Snippet: The antibodies used in our study were obtained from the following vendors: Trx, p21, and Chk1 antibodies were obtained from Santa Cruz Biotechnology (Santa Cruz, CA); RNR, MnSOD, and Chk2 antibodies were obtained from Millipore (Billerica, MA); poly(ADP-ribose) polymerase (PARP), p53, Article Title: Biphasic response of checkpoint control proteins in hyperoxia: Exposure to lower levels of oxygen induces genome maintenance genes in experimental baboon BPD Article Snippet: Chk2 antibody was obtained from Millipore (Billerica, MA); poly-ADP ribose polymerase (PARP), p53, phospho-p53 (ser15), and Article Title: Phosphorylation of nuclear Tau is modulated by distinct cellular pathways Article Snippet: Primary antibodies, usually incubated for 1 h at 37 °C, were specific for human Tau (Tau13, Santa Cruz, sc-21796, used at 1 μg/mL), GFP (Proteintech Europe, 66002–1-Ig, 7 μg/mL), calnexin (kind gift of Prof. Maurizio Molinari, IRB, Bellinzona, Switzerland, diluted 1:1,000), α-tubulin (Abcam, ab1825, 0.5 μg/mL or Cell Signaling, DM1A, diluted 1:500), pS 129 -H2A.X (Santa Cruz, sc-517348, 0.5 μg/mL), Article Title: Natural bioactive gallic acid shows potential anticancer effects by inhibiting the proliferation and invasiveness behavior in human embryonic carcinoma cells Article Snippet: Article Title: Replication stress and defective checkpoints make fallopian tube epithelial cells putative drivers of high-grade serous ovarian cancer. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies gH2AX Millipore Cat# 05-636; RRID: AB_309864 PAX8 Proteintech Cat# 10336; RRID: AB_2918972 CK8 TROMA-I -DSHB Cat# AB_531826; RRID: AB_531826 53BP1 Bethyl Labs Cat# A300-272A; RRID:AB_185520 53BP1 BioLegend Cat# 933002; RRID:AB_2820202 Foxj1 Millipore Sigma Cat# HPA005714; RRID:AB_1078902 Rad51 Santa Cruz Cat# SC-8349; RRID:AB_2253533 pRPA32 (S4/S8) Bethyl Labs Cat# A300-245A; RRID:AB_210547 pRPA32 (S33) Bethyl Labs Cat# |
![a., Plasmid DNA was replicated in Xenopus egg extracts in the presence of [α- 32 dATP] to yield supercoiled (SC) products. Plasmid DNA harboring a LacR array was also replicated in Xenopus egg extracts in the presence of [α- 32 dATP] to yield either (1) θ structures when forks were localized to the LacR barrier, and θ* structures after adding in aphidicolin and IPTG to simultaneously to induce uncoupling or (2) DNA breaks when replicated in the presence of aphidicolin or etoposide. b., Samples from a were separated on an agarose gel and visualized by autoradiography. c., Replication was performed as in a, but in the absence of [α- 32 dATP], see Supplemental Fig 5a. <t>pCHK1</t> and MCM10 proteins were detected by western blotting. d., Quantification of pCHK1 signal from c. Mean ± s.d. n=3. e., Plasmid DNA was replicated in Xenopus egg extracts. Forks were localized to the LacR barrier and uncoupling was induced by simultaneous addition of aphidicolin and IPTG. f., pCHK1 proteins from e were detected by western blotting. g., Quantification of pCHK1 signal from f. Mean ± s.d. n=3.](https://bio-rxiv-images-cdn.bioz.com/dois_ending_with_38/10__64898_slash_2026__03__22__666038/10__64898_slash_2026__03__22__666038___F5.large.jpg)
